effects of leukemia inhibitory factor on gp130 expression and rate of metaphase ii development during in vitro maturation of mouse oocyte

نویسندگان

آمنه محمدی روشنده

amaneh mohammadi roushandeh حسن حاجی حسینلو

hassan haji hosseinlou بهروز نیک نفس

behrouz niknafs راحله حلبیان

raheleh halabian احمد مهدی پور

چکیده

background: leukemia inhibitory factor (lif) is a 45-56 kda glycoprotein that has an important role in proliferation and embryo implantation. its effect on oocyte maturation and how to exert the function remained to be elucidated. methods: immature mice superovulated with human menopausal gonadotropin and germinal vesicle (gv) oocytes were obtained from ovary 48 hours after. gv oocytes were cultured in tissue culture medium 199 with 0, 100, 500 and 1,000 u/ml lif. cumulus expansion and in vitro maturation (ivm) rate were assessed after culture. for reverse transcriptase pcr, total rna from gv and metaphase ii (mii) oocytes were extracted by trizol reagent. the quantity and quality of rna were determined by spectrophotometry and electrophoresis, respectively. reverse transcription was performed by super script iii reverse transcriptase with 1 µg of total rna followed by dnase i treatment and heat inactivation. expression of gp130 was determined by rt-pcr. results: our results showed that cumulus expansion was improved with 1,000 u/ml in culture medium compared to others. gv breakdown and mii rate in groups with lif were higher than control group and were dose dependant. in 1,000 u/ml, lif rate of mii was significantly higher than control group (p<0.05). our results also showed that gp130 is expressed neither in gv nor in mii oocytes during ivm of mouse oocytes. conclusion: gp130 is expressed in human oocyte but not in mouse. our results suggest that in mouse, lif could affect the oocyte via another receptor or via cumulus cells however, further studies are warranted.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Effects of Leukemia Inhibitory Factor on gp130 Expression and Rate of Metaphase II Development during in vitro maturation of mouse oocyte

Background: Leukemia inhibitory factor (LIF) is a 45-56 kDa glycoprotein that has an important role in proliferation and embryo implantation. Its effect on oocyte maturation and how to exert the function remained to be elucidated. Methods: Immature mice superovulated with human menopausal gonadotropin and germinal vesicle (GV) oocytes were obtained from ovary 48 hours after. GV oocytes were cul...

متن کامل

Effects of leukemia inhibitory factor on gp130 expression and rate of metaphase II development during in vitro maturation of mouse oocyte.

BACKGROUND Leukemia inhibitory factor (LIF) is a 45-56 kDa glycoprotein that has an important role in proliferation and embryo implantation. Its effect on oocyte maturation and how to exert the function remained to be elucidated. METHODS Immature mice superovulated with human menopausal gonadotropin and germinal vesicle (GV) oocytes were obtained from ovary 48 hours after. GV oocytes were cul...

متن کامل

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...

the effect of e-64 on the developmental competence of sheep cocs during in vitro maturation

in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.

Melatonin Impact on In Vitro Development of Mouse Preantral Follicles and Oocyte Maturation

Introduction: Melatonin acts as an indirect antioxidant and is a powerful direct free radical scavenger and direct responses to melatonin in the gonads are detected. This study aims to investigate the influence of different doses of melatonin on preantral follicle development and oogenesis of in vitro cultured mouse ovarian follicles. Materials and Methods: Preantral follicles with diamet...

متن کامل

the effects of speech rate,prosodic features, and blurred speech on iranian efl learners listening comprehension

کلید واژه ها به زبان انگلیسی: effect of speech rate on listening comprehension, blurred speech,segmental and suprasegmental features,authentic speech,intelligibility, discrimination, omission, assimilation چکیده: سرعت مطالب شنیداری در کلام پیوسته بطور کلی همواره کابوسی بوده برای یادگیرنده های زبان دوم و بالاخص برای شنوندگان ایرانی. علی رغم عقل سلیم که کلام با سرعت کندتری فعالیتهای درک مطلب شن...

15 صفحه اول

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian biomedical journal

جلد ۱۴، شماره ۳، صفحات ۱۰۳-۱۰۷

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023